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EMD Millipore Amicon™ Ultra-0.5 Centrifugal Filter Units

Designed for protein purification, protein concentration and desalting. EMD Millipore Amicon™ Ultra-0.5 Centrifugal Filter Units feature reverse spin capability and are available at two MWCO levels. Constructed with Ultracel membranes, styrene/butadiene housing and polypropylene caps and tubes.

Manufacturer:  EMD Millipore UFC501024

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Catalog No. UFC501024


 

Specifications

Description

Filter Unit; Centrifugal; Millipore; Amicon; Ultra; Sample vol.: 0.5mL; Final concentrate vol.: 20μL; L: 4.99cm; Regenerated cellulose; Max force: 14,000xg for concentration spin; 1000xg for reverse spin; Ultracel-10 membrane; NMWL 10,000; 24/pk.

  • Concentrates 500μL down to 15μL
  • Concentration factor 25x to 30x
  • Fixed angle rotor: 14,000 × g for concentration spin; 1000 × g for reverse spin
  • High sample recovery (greater than 90% of dilute starting solution)
  • Fast processing time - 10 to 30 minutes
  • Reverse spin capability provides consistent recoveries
  • Enables the concentration of proteins with a membrane molecular weight limit of 3 kDa or 10 kDa

Applications:

  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates, removal of primer, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
  • Desalting and buffer exchange
Description & Specifications

Specifications

Regenerated Cellulose
Two microcentrifuge tubes
4.99cm
10,000 d
1cm2
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms; Purification of macromolecular components found in tissue culture extracts or cell lysates, removal of primer, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC: Desalting and buffer exchange
0.196 in.
14,000xG for concentration spin; 1000xG for reverse spin
24/Pk.