Embigin Monoclonal Antibody (G7.43.1), eBioscience™, Invitrogen™

Description

Description: This G7.43.1 monoclonal antibody reacts with mouse embigin (gp70), a single transmembrane glycoprotein that is a member of the Ig superfamily similar to basigin and EMMPRIN. It contains two Ig domains thought to be involved in cellular adhesion and tissue remodeling. Expression of embigin is high before day 10 in fetal development. In the adult it is expressed on myeloid cells and T cells but not B cells. During hematopoeisis, embigin is expressed on HSCs and CLPs (common lymphoid progenitors) but absent on pro- and pre-B cells. In recent studies Pax 5 has been shown to repress embigin expression. Applications Reported: This G7.43.1 antibody has been reported for use in flow cytometric analysis and immunohistology staining of frozen tissue sections. Applications Tested: This G7.43.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Embigin is a 327 amino acid regulatory protein belonging to the immunoglobulin superfamily class of CAMs, and has two Ig-like (immunoglobulin-like) V- type domains. In situ hybridization analysis shows that Embigin has specific carbohydrate epitopes and plays an important role in early postimplantation embryogenesis. A highly glycosylated transmembrane glycoprotein, Embigin acts as a regulator of cell/ECM interactions during development and homeostasis of normal adult tissues. Transfection studies suggest its role in promotion of integrin-mediated cell-substratum adhesion. Widely expressed in most of the tissues and embryonal carcinoma cells, prostate and mammary cancer cell lines, it may also help in the classification of human carcinomas.