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EGFR (ErbB1) [T790M] [L858R] Recombinant Human Protein, Invitrogen™ DFS Item

EGFR (ErbB1) [T790M] [L858R] Recombinant Human Protein

Manufacturer:  Life Technologies LSPV4879

Catalog No. PV4879



Description

Description

The epidermal growth factor receptor is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. The double T790M L858R mutant of EGFR displays resistance to Erlotinib (Tarceva) and sensitivity to HKI-272 and rapamycin combination therapy.
Specifications

Specifications

NP_005219.2; T790M L858R
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
90.6kDa
7.5
10μg
For research use only. Not for use in diagnostic procedures
Receptor tyrosine kinases
EGFR (ErbB1) [T790M] [L858R] Recombinant Human Protein
Kinase assay
Baculovirus
Amino acids 668-1210; T790M, L858R
150nmoles of phosphate transferred to Abl1 peptide substrate (EAIYAAPFAKKK) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.833μg/mL
GST Tag
0.38mg/mL
1956
EGFR (ErbB1) [T790M] [L858R] Recombinant Human Protein
65% by SDS-PAGE
–80°C
1 Each
EGFR (ErbB1) T790M L858R
EGFR T790M L858R was pre-diluted in enzyme dilution buffer and assayed in 20mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 1mM Na3VO4, 5mM β-glycerophosphate, 2mM DTT, 0.02% Triton™ X-100, 200μM ATP, 200μg/mL Abl1 peptide substrate (EAIYAAPFAKKK) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
EGF receptor (ErbB)
EGFR (ErbB1) [T790M] [L858R] Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.