Please login to your online account to display your discounted pricing

Thermo Scientific™ DyNAmo™ HS SYBR™ Green qPCR Kit


Perform sensitive, quantitative real-time PCR with this ready-to-use 2X master mix.

Manufacturer: thermo scientific™  F410XL

 View more versions of this product

 Disclaimers

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Catalog No. F410XL

  • / Each
 

Description & Specifications

Specifications

For Use With (Application) Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination
Includes 2x Master Mix (containing a hot start version of a modified Thermus brockianus DNA polymerase, SYBR Green I dye, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP), 50x ROX passive reference dye.
Quantity 1000 x 50µL Rxns (2500 x 20µL Rxns)
Storage Requirements Recommended storage temperature -20°C. Stable for six months from the packaging date

The master mix in Thermo Scientific DyNAmo HS SYBR Green qPCR Kit contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The modified DNA polymerase in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex.

ROX passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.

DyNAmo HS SYBR Green qPCR Kit contains all the reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The 2x master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex.

  • Specificity: DyNAmo HS SYBR Green master mix minimizes the formation of unwanted primer-dimers and extension of non-specifically bound primers during reaction setup
  • Sensitivity: Optimized master mix allows reproducible detection of low-copy number templates
  • Broad dynamic range: DyNAmo HS SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude
  • Convenience: 2x master mix, one tube only. ROX passive reference dye provided in a separate vial

Includes:

2x Master Mix (containing a hot start version of a modified Thermus brockianus DNA polymerase, SYBR Green I dye, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP), 50x ROX passive reference dye.

Recommended for:

Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination