Please login to your online account to display your discounted pricing

Thermo Scientific™ DyNAmo™ Flash SYBR™ Green qPCR Kit


Get fast qPCR results with high signal intensity, enhanced DNA-binding stability and processivity with excellent signal-to-noise ratio.

Manufacturer: thermo scientific™  F-415XL

 View more versions of this product

 Disclaimers

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Catalog No. F415XL

  • / Each
 

Description & Specifications

Specifications

For Use With (Application) Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination
Includes 2x master mix (contains hot start version of a modified Tbr DNA polymerase, SYBR Green I, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP), 50x ROX passive reference dye.
Quantity 1000 x 50µL Rxns (2500 x 20µL Rxns)
Storage Requirements Recommended storage temperature -20°C. Stable for six months from the packaging date.

DyNAmo Flash SYBR Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance. A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYBR Green qPCR Kit gives reliable quantification and early Cq values. Due to the high signal intensity, DyNAmo Flash SYBR Green qPCR provides an excellent signal-to-noise ratio.

DyNAmo Flash SYBR Green qPCR Kit is intended for highly specific quantitative PCR using a fast protocol. Optimized 2x master mix and a modified hot start DNA polymerase ensure reliable qPCR performance. DNA polymerase utilized in the kit incorporates a DNA-binding domain, which improves the physical stability of the polymerase-DNA complex.

  • Extremely fast protocols: Combined annealing and extension step only 15s
  • High specificity and sensitivity
  • High signal intensity
  • Elimination of carry-over contamination: dUTP in the master mix enables the use of UNG
  • Easy to use: Convenient 2x master mix and optimized protocols

Includes:

2x master mix (contains hot start version of a modified Tbr DNA polymerase, SYBR Green I, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP), 50x ROX passive reference dye.

Recommended for:

Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination