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Thermo Scientific™ DyNAmo™ Flash Probe qPCR Kit


Achieve extremely short cycling times and high amplification efficiency using the efficient Thermus brockianus DNA polymerase in this kit.

Manufacturer: thermo scientific™  F-455L

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 Disclaimers

† Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Catalog No. F455L

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Description & Specifications

Specifications

Description DyNAmo™ Flash Probe qPCR Kit
For Use With (Application) Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination
Includes 2x master mix (contains hot start Tbr DNA polymerase, optimized PCR buffer, MgCl2 and dNTP mix including dUTP), 50x ROX passive reference dye.
Quantity 200 x 50µL Rxns (500 x 20µL Rxns)
Storage Requirements Upon arrival, store all kit components at -20°C. When using the 2x master mix, the leftover thawed mix can be refrozen and stored at -20°C without affecting the performance of the kit.

DyNAmo Flash Probe qPCR Kit is the perfect choice for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. Performance of the DyNAmo Flash Probe qPCR Kit is based on an efficient hot start Thermus brockianus DNA polymerase. Sensitivity and the excellent amplification efficiency across a wide range of template concentrations guarantee reproducible results.

DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillary-based realtime PCR instruments.

DyNAmo Flash Probe qPCR Kit is designed for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. Performance of the DyNAmo Flash Probe qPCR Kit is based on an efficient hot start Thermus brockianus DNA polymerase.

  • Extremely fast protocols: combined annealing and extension step of only 15 s
  • Specific and sensitive detection of a wide range of template concentrations
  • Included dUTP allows the use of UNG for prevention of carry-over contamination
  • Convenient 2x master mix and optimized protocols
  • Validated with all major real-time PCR instruments
  • Licenced for qPCR

Includes:

2x master mix (contains hot start Tbr DNA polymerase, optimized PCR buffer, MgCl2 and dNTP mix including dUTP), 50x ROX passive reference dye.

Recommended for:

Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination