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Thermo Scientific™ DyNAmo™ ColorFlash SYBR™ Green qPCR kit

Minimize errors by providing a simple way to track pipetting of reaction components with this qPCR kit, a superior choice for fast, quantitative real-time analysis.

$104.21 - $1,698.90

Specifications

For Use With (Application) Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination
Includes 2x master mix with blue dye (contains hot start version of a modified Tbr DNA polymerase, SYBR Green I, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP), 40x sample buffer solution with yellow dye, 50x ROX passive reference dye.
Storage Requirements Upon arrival, store all kit components at -20°C. When using the 2x master mix, the leftover thawed mix can be refrozen and stored at -20°C without affecting the performance of the kit. The yellow sample buffer solution is stable and can be stored at +4°C, but storage at −20°C with the other kit components is recommended. Stable for six months from the packaging date.
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 Disclaimers

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

F416L

 
thermo scientific™
F-416L
200 x 50µL Rxns (500 x 20µL Rxns) Each for $400.05

F416S

 
thermo scientific™
F-416S
40 x 50µL Rxns (100 x 20µL Rxns) Each for $104.21

F416XL

 
thermo scientific™
F-416XL
1000 x 50µL Rxns (2500 x 20µL Rxns) Each for $1,698.90
Description & Specifications

Specifications

For Use With (Application) Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination
Includes 2x master mix with blue dye (contains hot start version of a modified Tbr DNA polymerase, SYBR Green I, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP), 40x sample buffer solution with yellow dye, 50x ROX passive reference dye.
Storage Requirements Upon arrival, store all kit components at -20°C. When using the 2x master mix, the leftover thawed mix can be refrozen and stored at -20°C without affecting the performance of the kit. The yellow sample buffer solution is stable and can be stored at +4°C, but storage at −20°C with the other kit components is recommended. Stable for six months from the packaging date.

DyNAmo ColorFlash SYBR Green qPCR Kit offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction setup, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

Highlights

  • Colored reagents minimize the risk of pipetting errors during reaction set up
  • Especially beneficial when using white reaction vessels
  • Extremely fast protocols due to combined annealing and extension step of only 15 s
  • Specific and sensitive detection of a wide range of template concentrations
  • dUTP included in the 2x master mix allows the use of UNG for prevention of carry-over contamination