Thermo Scientific™ DyNAmo ColorFlash SYBR™ Green qPCR Kit
Offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting.
Manufacturer: Thermo Scientific™ F416S
The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction setup, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.
- Colored reagents minimize the risk of pipetting errors during reaction set up, especially beneficial when using white reaction vessels
- Extremely fast protocols due to combined annealing and extension step of only 15 sec.
- Specific and sensitive detection of a wide range of template concentrations
- dUTP included in the 2X master mix allows the use of UNG for prevention of carry-over contamination
|Real time PCR (qPCR), Fast qPCR, qPCR using SYBR green dye, RT-qPCR using SYBR green dye|
|Store at -20°C.|
|5700 Sequence Detection System, 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, Bio-Rad CFX384 Touch™, BioRad CFX96™, Cepheid SmartCycler, Corbett Rotor-Gene, Eppendorf MasterCycler RealPlex, MJ Chromo4, MJ Opticon, QuantStudio™, Roche LightCycler 480, StepOne™ System, StepOnePlus™ System, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA™ 7 System|
|1 x 1mL DyNAmo ColorFlash SYBR Green qPCR Mix (2X); 1 x 50μL 50X ROX dye, 25μM; 1 x 200μL 40X sample buffer solution with yellow dye|
|40 x 50µL Rxns (100 x 20µL Rxns)|
|Detection Method: SYBR; PCR Method: qPCR|