Please login to your online account to display your discounted pricing

Thermo Scientific™ DyNAmo™ ColorFlash Probe qPCR Kit


Perform fast, quantitative real-time analysis and easily set up qPCR reactions, minimize errors, and track pipetting with colored reagents in this qPCR kit.

Manufacturer: thermo scientific™  F-456XL

 View more versions of this product

 Disclaimers

† Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Catalog No. F456XL

  • / Each
 

Description & Specifications

Specifications

Description DyNAmo™ ColorFlash Probe qPCR Kit
For Use With (Application) Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination
Includes 2x master mix with blue dye (contains hot start version of a modified Tbr DNA polymerase, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP), 40x sample buffer solution with yellow dye, 50x ROX passive reference dye.
Quantity 1000 x 50µL Rxns (2500 x 20µL Rxns)
Storage Requirements Upon arrival, store all kit components at -20°C. When using the 2x master mix, the leftover thawed mix can be refrozen and stored at -20C without affecting the performance of the kit. The yellow sample buffer solution is stable and can be stored at +4°C, but storage at -20°C with the other kit components is recommended. Stable for six months from the packaging date.

DyNAmo ColorFlash Probe qPCR Kit offers equal performance to the DyNAmo Flash Probe qPCR Kit. In addition, it incorporates an innovative multicolor system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction set up, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

DyNAmo ColorFlash Probe qPCR Kit is a superior choice for fast, quantitative real-time analysis. The kit offers excellent performance in detection and quantification of DNA sequences from various sources. It utilizes an innovative multi-color system: the 2x master mix contains a blue dye, and a separate sample buffer contains a yellow dye. When both the master mix and sample DNA have been pipetted into, the reaction mixture, it turns green. DyNAmo ColorFlash Probe qPCR Kit is designed to ease qPCR setup and minimize errors by providing a simple way to track pipetting of reaction components.

  • Minimized risk of pipetting errors during reaction setup
  • Also beneficial when using white reaction vessels
  • Extremely fast protocols due to combined annealing and extension step of only 15 seconds
  • Specific and sensitive detection of a wide range of template concentrations
  • dUTP included in the 2x Master Mix allows the use of UNG for prevention of carry-over contamination
  • Licensed for qPCR

Includes:

2x master mix with blue dye (contains hot start version of a modified Tbr DNA polymerase, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP), 40x sample buffer solution with yellow dye, 50x ROX passive reference dye.

Recommended for:

Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination