missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Thermo Scientific™ DyLight™ Sulfhydryl-Reactive Fluorescent Dyes

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of maleimide-activated DyLight Dyes.

$453.38 - $611.64

Specifications

Quantity 1mg
View More Specs


Products ${productFamilyLength}
Catalog Number Mfr. No. Description Price Quantity  
Catalog Number Mfr. No. Description Price Quantity  
PI46600 Thermo Scientific™
46600
DyLight 405 Maleimide Each for $459.93
PI46602 Thermo Scientific™
46602
DyLight 488 Maleimide Each for $456.66
PI46608 Thermo Scientific™
46608
DyLight 594 Maleimide Each for $455.02
PI46613 Thermo Scientific™
46613
DyLight 633 Maleimide Each for $453.38
PI46618 Thermo Scientific™
46618
DyLight 680 Maleimide Each for $453.38
PI46621 Thermo Scientific™
46621
DyLight 800 Maleimide Each for $456.66
PI62290 Thermo Scientific™
62290
DyLight 550 Maleimide Each for $453.38
PI62295 Thermo Scientific™
62295
DyLight 650 Maleimide Each for $459.93
PI62298 Thermo Scientific™
62298
DyLight 755 Maleimide Each for $611.64
Specifications

Description

Thermo Scientific™ Sulfhydryl-Reactive DyLight Dyes are maleimide-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as probes for cellular imaging and other fluorescence detection methods.

The Thermo Scientific™ DyLight™ Dyes have absorption spectra ranging from 350 to 770nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor™ or Cy™ Dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight Reagents allows a high fluor-to-protein ratio without precipitation during conjugation.
The sulfhydryl-reactive dyes contain maleimide groups that react predominantly with free –SH groups at pH 6.5-7.5, forming a stable thioether bond. At pH 7, the maleimide group is ~1000 times more reactive toward a free sulfhydryl than to an amine.1At pH values > 7.5, reactivity toward primary amines increases and hydrolysis of the maleimide group can occur.

Highlights:

  • Specific — maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
  • Efficient labeling methods — well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
  • Optimized antibody labeling procedure —complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:

  • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
  • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies
Description & Specifications

Specifications

1mg