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Thermo Scientific™ DyLight™ Sulfhydryl-Reactive Fluorescent Dyes

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of maleimide-activated DyLight Dyes.

$445.20 - $600.60

Specifications

Quantity 1mg
View More Specs
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PI46600

 
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DyLight 405 Maleimide Each for $445.20

PI46602

 
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DyLight 488 Maleimide Each for $445.20

PI46608

 
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46608
DyLight 594 Maleimide Each for $445.20

PI46613

 
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46613
DyLight 633 Maleimide Each for $445.20

PI46618

 
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46618
DyLight 680 Maleimide Each for $445.20

PI46621

 
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46621
DyLight 800 Maleimide Each for $445.20

PI62290

 
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62290
DyLight 550 Maleimide Each for $445.20

PI62295

 
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62295
DyLight 650 Maleimide Each for $445.20

PI62298

 
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DyLight 755 Maleimide Each for $600.60
Description & Specifications

Specifications

Quantity 1mg

Thermo Scientific™ Sulfhydryl-Reactive DyLight Dyes are maleimide-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as probes for cellular imaging and other fluorescence detection methods.

The Thermo Scientific™ DyLight™ Dyes have absorption spectra ranging from 350 to 770nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor™ or Cy™ Dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight Reagents allows a high fluor-to-protein ratio without precipitation during conjugation.
The sulfhydryl-reactive dyes contain maleimide groups that react predominantly with free –SH groups at pH 6.5-7.5, forming a stable thioether bond. At pH 7, the maleimide group is ~1000 times more reactive toward a free sulfhydryl than to an amine.1At pH values > 7.5, reactivity toward primary amines increases and hydrolysis of the maleimide group can occur.

Highlights:

  • Specific — maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
  • Efficient labeling methods — well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
  • Optimized antibody labeling procedure —complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:

  • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
  • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies