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Thermo Scientific™ DyLight™ Near-IR Specialty Dyes

Label amine-containing molecules for fluorescence imaging or flow cytometry with these benzopyrillium- and cyanine-based, infrared-emitting (633 or 700 laser) NHS ester dyes.

$316.05 - $367.50

Specifications

Quantity 1mg
Sufficient For 1 use to label a total of 7mg of IgG using typical conditions
View More Specs
Products
Catalog Number Mfr. No. Description Price Quantity    

PI46646

 
thermo scientific™
46646
DyLight 675-B1 NHS Ester Each for $360.15

PI46647

 
thermo scientific™
46647*
DyLight 675-B2 NHS Ester Each for $367.50

PI46648

 
thermo scientific™
46648
DyLight 675-B3 NHS Ester Each for $360.15

PI46649

 
thermo scientific™
46649
DyLight 675-B4 NHS Ester Each for $360.15

PI53003

 
thermo scientific™
53003
DyLight 679-C5 NHS Ester Each for $316.05

PI53023

 
thermo scientific™
53023
DyLight 690- B1 Free Acid Each for $321.30

PI53026

 
thermo scientific™
53026
DyLight 690-B2 NHS Ester Each for $360.15

PI53028

 
thermo scientific™
53028
DyLight 700-B1 NHS Ester Each for $360.15

PI53030

 
thermo scientific™
53030*
DyLight 700-B2 NHS Ester Each for $367.50

PI53032

 
thermo scientific™
53032
DyLight 730-B1 NHS Ester Each for $360.15

PI53033

 
thermo scientific™
53033
DyLight 730-B2 NHS Ester Each for $360.15

PI53036

 
thermo scientific™
53036
DyLight 730-B3 NHS Ester Each for $360.15

PI53037

 
thermo scientific™
53037
DyLight 730-B4 NHS Ester Each for $360.15

PI53040

 
thermo scientific™
53040
DyLight 747-B2 NHS Ester Each for $360.15

PI53041

 
thermo scientific™
53041
DyLight 747-B3 NHS Ester Each for $360.15

PI53042

 
thermo scientific™
53042
DyLight 747-B4 NHS Ester Each for $360.15

PI53054

 
thermo scientific™
53054
DyLight 775-B2 NHS Ester Each for $360.15

PI53055

 
thermo scientific™
53055
DyLight 775-B3 NHS Ester Each for $360.15

PI53064

 
thermo scientific™
53064
DyLight 780-B1 NHS Ester Each for $360.15

PI53065

 
thermo scientific™
53065
DyLight 780-B2 NHS Ester Each for $360.15

PI53066

 
thermo scientific™
53066
DyLight 780-B3 NHS Ester Each for $360.15

PI53067

 
thermo scientific™
53067
DyLight 830-B2 NHS Ester Each for $360.15

PI85150

 
thermo scientific™
85150
DyLight 775-B4 NHS Ester Each for $321.30
Description & Specifications

Specifications

Quantity 1mg
Sufficient For 1 use to label a total of 7mg of IgG using typical conditions

Thermo Scientific™ DyLight Near Infrared Specialty Dyes offer a broad spectrum of options for labeling molecules with tissue-penetrating near infrared (NIR) fluorescence signatures.

DyLight NIR Dyes are a family of labeling agents that can be used for bright fluorescence detection in cell-based imaging or in vivo imaging applications. NIR dyes can be selected based upon their characteristic excitation and emission properties or relative hydrophilicity/hydrophobicity attributes. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetrating ability in vivo, while more hydrophilic dyes have less nonspecific binding potential. Each dye contains an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation. NIR dyes are best for imaging through tissues and away from indigenous fluorescent biomolecule interference or quenching. DyLight Near Infrared Dyes represent the largest selection of fluorescent labels that are commercially available.

Highlights:

  • Large selection–the largest family of dyes available for NIR fluorescence applications
  • NHS ester reactive group–allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
  • Broad spectrum of water solubilities–choose from hydrophilic to hydrophobic dyes to optimize the right dye label for the best performance in a given application
  • NIR dyes avoid background interference–DyLight NIR Dyes avoid fluorescence interference or quenching effects from biomolecules present in samples
  • Excellent signal penetration through cells and tissues–DyLight NIR Dyes provide the optimal window for excitation and emission for in vivo imaging applications

Criteria to consider when choosing a DyLight NIR Specialty Dye:

  • Excitation and emission wavelengths–choose the best dye to match the excitation and emission capabilities of your instrument
  • Water solubility–choose a DyLight NIR Dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.
  • DyLight Dye selection–the broad selection of NIR dyes allows a number of candidate dyes to be tested in a given application for optimal performance

Recommended for:

In vivo or ex vivo imaging; Tumor imaging with labeled peptides; NIR fluorescence (NIRF) imaging of labeled silica nanoparticles; NIR in vitro imaging and characterization; Determination of thermal stability; Cytotoxicity assays; Molecular imaging; UV-VIS-NIR spectroscopy; Fluorescence correlation spectroscopy; MRI applications; DNA sequencing; Primer labeling for PCR; 2-D gel electrophoresis; Flow cytometry/fluorescence-activated cell sorting (FACS); Laser scanning confocal microscopy