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Thermo Scientific™ DyLight™ Amine-Reactive Fluorescent Dyes


Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.

Manufacturer: thermo scientific™  46427

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Catalog No. PI46427

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Description & Specifications

Specifications

Description DyLight 350 NHS Ester
Formulation NHS ester activated proprietary blue fluorescent dye
Sufficient For 5 uses to label a total of 3mg of IgG using typical conditions
Quantity 5 x 65µg

Thermo Scientific™ Amine-Reactive DyLight Dyes are NHS ester-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as molecular probes for cellular imaging and other fluorescence detection methods.

The DyLight Dyes exhibit higher fluorescence intensity and photostability than Alexa™, CyDye™ and LI-COR™ Dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9). Additionally, the water solubility of the DyLight Dyes allows a high dye-to-protein ratio without precipitation during conjugation.

Highlights:

  • Brightness: high-fluorescence intensity provides outstanding sensitivity and requires less conjugate

  • Photostability: exceptional resistance to photobleaching enables fluorescence imaging under the most demanding conditions (e.g., structured illumination, 4pi microscopy)

  • Instrument compatibility: emission spectra match the principal output wavelengths of common fluorescence instrumentation