TCR DO11.10 Monoclonal Antibody (KJ1-26 (KJ126, KJI-26, KJI26)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

Description: The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-A^d major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity. Applications Reported: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been reported for use in flow cytometric analysis. Applications Tested: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been tested by flow cytometric analysis of DO.11 cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.