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Thermo Scientific™ DNase I, RNase-free, HC (50 U/µL)

An endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

$80.65 - $82.66

Specifications

Quantity 1000U
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Products 2
Catalog Number Mfr. No. Quantity Concentration Price Quantity  
Catalog Number Mfr. No. Quantity Concentration Price Quantity  
FEREN0521 View Documents Thermo Scientific™
EN0521
1000U 1U/μL Each for $80.65
 
FEREN0523 View Documents Thermo Scientific™
EN0523
1000U 50U/μL Each for $82.66
 
Description

Description

Dnase I, Rnase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.

  • In the presence of Mg2+, Dnase I cleaves each strand of dsDNA independently in a statistically random fashion.
  • In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides.

Highlights

  • Recombinant enzyme
  • Purified from non-animal host with a lower level of intrinsic RNases
Specifications

Specifications

1000U