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Thermo Scientific™ DNA Polymerase I

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

Manufacturer:  Thermo Scientific™ EP0042

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Catalog No. FEREP0042


 

Specifications

Description

DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.

Highlights

  • Incorporates modified nucleotides(e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
  • Active in multiple buffers, including restriction enzyme, PCR, and RT buffers

Applications

  • DNA labeling by nick-translation in conjunction with DNase
  • Second-strand synthesis of cDNA in conjunction with RNase
Description & Specifications

Specifications

10U/µL
metal chelators, PPi, Pi (at high concentrations) (5)
2,500U
E.coli cells with a cloned polA gene
103kDa monomer