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Thermo Scientific™ DNA Polymerase I


DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

Manufacturer: thermo scientific™  EP0041

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Catalog No. FEREP0041

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Description & Specifications

Specifications

Concentration 10U/µL
Inhibitors metal chelators, PPi, Pi (at high concentrations) (5)
Source E.coli cells with a cloned polA gene
Molecular Weight 103kDa monomer
Units 500U

DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.

Highlights

  • Incorporates modified nucleotides(e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
  • Active in multiple buffers, including restriction enzyme, PCR, and RT buffers

Applications

  • DNA labeling by nick-translation in conjunction with DNase
  • Second-strand synthesis of cDNA in conjunction with RNase