Thermo Scientific™ DNA Polymerase I
DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.
Manufacturer: Thermo Scientific™ EP0041
DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.
- Incorporates modified nucleotides(e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
- Active in multiple buffers, including restriction enzyme, PCR, and RT buffers
- DNA labeling by nick-translation in conjunction with DNase
- Second-strand synthesis of cDNA in conjunction with RNase
|metal chelators, PPi, Pi (at high concentrations) (5)|
|E.coli cells with a cloned polA gene|