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DNA-PK Human Protein DFS Item

DNA-PK Human Protein

Manufacturer:  Life Technologies LSPV5866

Catalog No. PV5866



Description

Description

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signalling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases.
Specifications

Specifications

0.12mg/mL
DNA-PK
DNA-PK Human Protein
10μg
For research use only. Not for use in diagnostic procedures
PRKDC
DNA-PK was pre-diluted in enzyme dilution buffer and assayed in 50mM HEPES (pH 7.5), 10mM MgCl2, 100mM KCl, 0.2mM EGTA, 0.1mM EDTA, 1mM DTT, 10μg/mL DNA-PK activator (PV5884), 0.08mg/mL BSA, 200μM ATP, 2μg/mL DNA-PK substrate (EPPLSQEAFADLWKK) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
PIKK
DNA-PK Human Protein
Human
Frozen
600kDa
70% by SDS-PAGE
–80°C
Lipid kinases
DNA-PK Human Protein
Kinase assay
Baculovirus
Full length
290nmoles of phosphate transferred to DNA-PK substrate (EPPLSQEAFADLWKK) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.83μg/mL
Documents

For Research Use Only. Not for use in diagnostic procedures.