Desmin, eFluor® 660, Clone: DE-U-10, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Life Technologies LS50974782
Description: The monoclonal antibody DE-U-10 recognizes desmin. Desmin is the protein subunit of class-III intermediate filaments. Desmin is found predomitly in skeletal, cardiac, and smooth muscle. Desmin plays a role in muscle cell development and diffeThis DE-U-10 antibody has been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. Desmin (∽53 kDa) exhibits a high degree of tissue specificity, its expression being predomitly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
|PBS with 0.09% sodium azide; pH 7.2|
|P48675, P02540, P31001, P17661, P02542, O62654|
|CMD1I, CSM2, DES, FLJ12025|
|Bovine, Rat, Chicken, Feline, Goat, Hamster, Human, Mouse, Ovine, Porcine, Rabbit|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)|
|4° C, store in dark, DO NOT FREEZE!|
|100328890, 100764990, 101097010, 101117308, 102180712, 13346, 1674, 280765, 395906, 396725, 64362|
For Research Use Only.