DAPK2 Recombinant Human Protein

Manufacturer: Life Technologies LSPV3614

Catalog No. PV3614

Quantity

Description

DAPK2 is a calcium⁄calmodulin-dependent kinase that acts as a positive regulator of apoptosis, and is expressed in many tissue types.

Specifications

Accession Number	NP_055141
For Use With (Application)	Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis, target and lead identification and validation
Molecular Weight	47.9kDa
Purity	85% by SDS-PAGE
Storage Requirements	–80°C
Packaging	1 Each
Gene Alias	DAPK2, DRP-1
Assays	DAPK2 was pre-diluted in enzyme dilution buffer and assayed in 12.5mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 4.8μM calmodulin, 0.5mM CaCl2, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 167μg/mL MLCK peptide substrate (KKRAARATSDVFA-NH2) and trace [32P]-γ-ATP for 10 minutes at 30°C
Protein Family	Kinase and inhibitors
KinaseFamily	DAPK
Specific Reactivity	18nmoles of phosphate transferred to MLCK peptide substrate (KKRAARATSDVFA-NH2) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 8.33μg/mL
Protein Tag	His Tag

Concentration	0.34mg/mL
Gene ID (Entrez)	23604
Name	DAPK2 Recombinant Human Protein
Quantity	10μg
Regulatory Status	For research use only. Not for use in diagnostic procedures
Protein Subtype	Serine/threonine kinase
Common Name	DAPK2 Recombinant Human Protein
Validated Application	Kinase assay
Expression System	Baculovirus
Product Type	DAPK2 Recombinant Human Protein
Host Species	Human

For Research Use Only. Not for use in diagnostic procedures.

Description