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Thermo Scientific™ Lab Vision™ Cyclin B1 Ab-2, Rabbit Polyclonal Antibody


Ensure accurate, reproducible results in immunohistochemistry, western blotting and immunoprecipitation experiments.

Manufacturer: thermo scientific™ lab vision™  RB008P

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Catalog No. RB008P

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Description & Specifications

Specifications

Antigen Cyclin B1 Ab-2
Applications Immunoprecipitation
Classification Polyclonal
Clone Polyclonal
Concentration 1mg/mL
Conjugate Unlabeled
Cross Reactivity Human
Cross Reactivity Mouse
Format Concentrated
Formulation Purified Ab with BSA and Azide
Host Species Rabbit
Immunogen A synthetic peptide derived from the C-terminus of human cyclin B1
Isotype IgG
Quantity 1mL
Regulatory Status RUO
Research Discipline Cell Cycle
Type Primary

Ensure accurate, reproducible results in Immunohistochemistry, Western Blotting and Immunoprecipitation experiments with Thermo Scientific™ Cyclin B1 Ab-2, Rabbit Polyclonal Antibody.

In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Host Species: Rabbit

Isotype: IgG

Species Reactivity: Human, Mouse and Rat. Others not known.

Epitope: C-terminal

Immunogen: A synthetic peptide derived from the C-terminus of human cyclin B1

Molecular Weight: 62 kDa

Positive Control: Jurkat, Ls174T, HeLa cells; Breast CA

Cellular Localization: Cytoplasmic

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting
  • Immunoprecipitation (Native and denatured)

Note:

USA: RUO; Int'l: RUO