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Thermo Scientific™ Lab Vision™ Cyclin B1 Ab-2, Rabbit Polyclonal Antibody

Ensure accurate, reproducible results in immunohistochemistry, western blotting and immunoprecipitation experiments.

$227.33 - $1,134.47

Specifications

Antigen Cyclin B1 Ab-2
Clone Polyclonal
Concentration 1mg/mL
Applications Immunoprecipitation
Classification Polyclonal
View More Specs
Products
Catalog Number Mfr. No. Formulation Quantity Price Quantity    

RB008P

 
thermo scientific™ lab vision™
RB008P
Purified Ab with BSA and Azide 1mL Each for $1,044.24

RB008P0

 
thermo scientific™ lab vision™
RB008P0
Purified Ab with BSA and Azide 100µL Each for $227.33

RB008P1

 
thermo scientific™ lab vision™
RB008P1
Purified Ab with BSA and Azide 500µL Each for $664.86

RB008P1ABX

 
thermo scientific™ lab vision™
RB008P1ABX
Ab without BSA/Azide (Frozen) 500µL Each for $742.71

RB008PABX

 
thermo scientific™ lab vision™
RB008PABX
Ab without BSA/Azide (Frozen) 1mL Each for $1,134.47
Description & Specifications

Specifications

Antigen Cyclin B1 Ab-2
Clone Polyclonal
Concentration 1mg/mL
Applications Immunoprecipitation
Classification Polyclonal
Conjugate Unlabeled
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Human
Cross Reactivity Mouse
Format Concentrated
Host Species Rabbit
Regulatory Status RUO
Research Discipline Cell Cycle
Type Primary
Immunogen A synthetic peptide derived from the C-terminus of human cyclin B1
Isotype IgG

Ensure accurate, reproducible results in Immunohistochemistry, Western Blotting and Immunoprecipitation experiments with Thermo Scientific™ Cyclin B1 Ab-2, Rabbit Polyclonal Antibody.

In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Host Species: Rabbit

Isotype: IgG

Species Reactivity: Human, Mouse and Rat. Others not known.

Epitope: C-terminal

Immunogen: A synthetic peptide derived from the C-terminus of human cyclin B1

Molecular Weight: 62 kDa

Positive Control: Jurkat, Ls174T, HeLa cells; Breast CA

Cellular Localization: Cytoplasmic

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting
  • Immunoprecipitation (Native and denatured)

Note:

USA: RUO; Int'l: RUO