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Thermo Scientific™ Lab Vision™ Cyclin B1 Ab-2, Rabbit Polyclonal Antibody


Ensure accurate, reproducible results in immunohistochemistry, western blotting and immunoprecipitation experiments.

Manufacturer: thermo scientific™ lab vision™  RB008P0

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Catalog No. RB008P0

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Description & Specifications

Specifications

Clone Polyclonal
Host Species Rabbit
Immunogen A synthetic peptide derived from the C-terminus of human cyclin B1
Cross Reactivity Human
Cross Reactivity Mouse
Quantity 100µL
Format Concentrated
Antigen Cyclin B1 Ab-2
Isotype IgG
Applications Immunoprecipitation
Regulatory Status RUO
Conjugate Unlabeled
Formulation Purified Ab with BSA and Azide
Type Primary
Classification Polyclonal
Concentration 1mg/mL
Research Discipline Cell Cycle
Item Description 100uL; 1mg/mL; Purified w/BSA, azide

Ensure accurate, reproducible results in Immunohistochemistry, Western Blotting and Immunoprecipitation experiments with Thermo Scientific™ Cyclin B1 Ab-2, Rabbit Polyclonal Antibody.

In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Host Species: Rabbit

Isotype: IgG

Species Reactivity: Human, Mouse and Rat. Others not known.

Epitope: C-terminal

Immunogen: A synthetic peptide derived from the C-terminus of human cyclin B1

Molecular Weight: 62 kDa

Positive Control: Jurkat, Ls174T, HeLa cells; Breast CA

Cellular Localization: Cytoplasmic

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting
  • Immunoprecipitation (Native and denatured)

Note:

USA: RUO; Int'l: RUO