Thermo Scientific™ pH Gradient Buffers
Robust reproducible gradients that are applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.
Manufacturer: Thermo Scientific™ 085349
Delivers robust reproducible gradients with the Thermo Scientific™ pH buffer platform that is applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.
The pH gradient platform saves time in method development and facilitates method transfer to QA/QC for a wide range of MAb charge variants through a generic LC-based approach to MAb characterization.pH buffer solutions can be used to generate highly reproducible, linear pH gradients in cation exchange chromatography.
- Robust, reproducible gradients
- Predict biomolecule pI
- Simple method optimization
- Easily automated
- Applicable to the majority of MAbs
Unlike traditional cation exchange chromatography using salt gradients, it is possible to predict the pI and the expected retention of the charge variants and use a narrow pH range to get a higher resolution separation.
|pH Gradient Buffer Kit|
|Buffer A (250mL) and Buffer B (250mL)|
|5.6 to 10.2|
|CX-1 pH Gradient Buffer Kit|
|LC columns and systems|