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Corning™ BioCoat™ Fibronectin HTS Multiwell Angiogenesis

Provides a standard format to increase the quality and reproducibility of in vitro endothelial cell migration assays

$462.43 - $3,251.82

Specifications

Pore Size 3μm
Type Multiwell Insert Plate
For Use With (Application) High Throughput Screening
Surface Coating Fibronectin
View More Specs
Includes:  A Falcon 24- and 96-Multiwell™ insert plate containing a fluorescence blocking, microporous polyethylene terephthalate (PET) membrane evenly coated with human fibronectin, an untreated receiver plate, and a lid

 Alert

In October 2012, Corning acquired the majority of the Becton Dickinson Discovery Labware business. In 2015, the integration of the Biologics portion of the Discovery Labware brands (including Matrigel™, BioCoat™, FluoroBlok™ and PureCoat™) into their growing family of Corning Life Sciences brands will be complete. You will notice changes on their product and packaging artwork, but the products themselves and the catalog numbers will remain the same.

Products
Catalog Number Mfr. No. No. of Wells Quantity Culture Area Price Quantity    

08-774-370

 
corning™
354144
24 5 0.3 cm2 Case of 5 Each for $2,004.49

08-774-366

 
corning™
C354143
24 1 0.3 cm2 Each for $462.43

08-774-399

 
corning™
C354148
96 5 0.08 cm2 Case of 5 Each for $3,251.82

08-774-398

 
corning™
C354147
96 1 0.08 cm2 Each for $715.44
Description & Specifications

Specifications

Pore Size 3μm
Type Multiwell Insert Plate
For Use With (Application) High Throughput Screening
Surface Coating Fibronectin

  • Combines the fluorescence blocking capabilities of the BD Falcon™ FluoroBlok™ membrane with the benefits of an extracellular matrix protein
  • Provides a predictive in vitro model for screening pro- and anti-angiogenic agents
  • Eliminates tedious manual cell scraping and counting
  • Fibronectin concentration and coating procedure have been optimized so the pores of the membrane are not obstructed, allowing endothelial cells to attach to the membrane and freely migrate toward an angiogenic stimulus in the receiver plate
  • Cells may be labeled with a fluorescent dye either pre- or post-migration and quantified using a fluorescence microplate reader
  • Light-tight membrane blocks the transmission of light within the 490 to 700nm range, shielding fluorescently labeled cells in the top of the insert from detection by a bottom-reading microplate reader and allowing for real-time detection without dismantling the insert
  • Tested for the ability to support migration of HUVEC (human umbilical vein endothelial cells) in response to VEGF (vascular endothelial cell growth factor)
  • Automation compatible
  • Stable for at least three months when stored at -20°C