Collagen IV, Alexa Fluor 488, clone: 1042, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Life Technologies LS53987182
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.Description: This monoclonal antibody 1042 recognizes human collagen Type IV. Collagens are the main components of the extracellular matrix and provide the tensile strength to tissues. There are four main types of collagen (I, II, III and IV) which contribute to the connective tissue. Expression of type IV is restricted to the basal lamina. It contains two alpha-1 and one alpha-2-like chains. Collagen Type IV is associated with disorders such as Alport syndrome and Goodpasture's syndrome.Applications Reported: This 1042 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 1042 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|IgG2b, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)|
|Alexa Fluor 488|
For Research Use Only.