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Thermo Scientific™ Lab Vision™ Collagen II Ab-3, Mouse Monoclonal Antibody


Obtain accurate, reproducible results in immunohistochemistry and immunofluorescence experiments with  Thermo Scientific™ Collagen II Ab-3, Mouse Monoclonal Antibody.

Manufacturer: thermo scientific™ lab vision™  MS306P1ABX

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Catalog No. MS306P1ABX

  • / Each
 

Description & Specifications

Specifications

Regulatory Status RUO
Clone 6B3
Cross Reactivity Chicken
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Salamander
Host Species Mouse
Antigen Collagen II Ab-3
Applications Immunofluorescence
Applications Immunohistochemistry (Paraffin)
Immunogen Purified type II collagen; obtained from the adult chicken sternum by limited pepsinization
Quantity 100µL
Format Concentrated
Isotype IgG1
Formulation wWithout BSA/Azide (Frozen)
Research Discipline Extracellular Matrix
Type Primary
Classification Monoclonal
Concentration 1mg/mL
Conjugate Unlabeled
Item Description 100uL; 1mg/mL; Purified w/o BSA, azide

Obtain accurate, reproducible results in immunohistochemistry and immunofluorescence experiments with  Thermo Scientific™ Collagen II Ab-3, Mouse Monoclonal Antibody.

Host Species: Mouse

Clone: 6B3

Isotype: IgG1

Species Reactivity: Human, Mouse, Chicken and Salamander. Others not known.

Epitope: Triple helix of type II collagen

Immunogen: Purified type II collagen; obtained from the adult chicken sternum by limited pepsinization

Positive Control: Cartilage in lung or fetus

Cellular Localization: Cartilage matrix

In Western blotting, Ab-3 reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin-digested type II collagen. Immunoblotting of CNBr peptides of collagen II shows that MAb 6B3 reacts with CB 11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. In Western blotting (but not ELISA), Ab-3 recognizes alpha1(II) as well as alpha3(XI) chain both of which have identical primary structure.

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting (Not verified)
  • Immunofluorescence

Note:

USA: RUO; Int'l: RUO