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Thermo Scientific™ Lab Vision™ Chk2 Ab-1, Mouse Monoclonal Antibody


Ensure accurate, reproducible results in western blotting, immunofluorescence and immunoprecipitation experiments with Thermo Scientific™ Chk2 Ab-1, Mouse Monoclonal Antibody.

Manufacturer: thermo scientific™ lab vision™  MS1330PABX

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Catalog No. MS1330PABX

  • / Each
 

Description & Specifications

Specifications

Antigen Chk2 Ab-1
Applications Immunofluorescence
Applications Immunoprecipitation
Applications Western Blotting
Classification Monoclonal
Clone 2CHK01; same as DCS-293
Concentration 1mg/mL
Conjugate Unlabeled
Cross Reactivity Human
Format Concentrated
Host Species Mouse
Immunogen Recombinant Chk2 protein
Isotype IgG2a kappa
Quantity 200µL
Regulatory Status RUO
Research Discipline Cell Cycle
Type Primary

Ensure accurate, reproducible results in western blotting, immunofluorescence and immunoprecipitation experiments with Thermo Scientific™ Chk2 Ab-1, Mouse Monoclonal Antibody.

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phophorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

Host Species: Mouse

Clone: 2CHK01; same as DCS-293

Isotype: IgG1/κ

Species Reactivity: Human. Others not known.

Epitope: Not determined

Immunogen: Recombinant Chk2 protein

Molecular Weight: ∽70 kDa

Positive Control: HeLa cells

Cellular Localization: Nuclear

Recommended for:

  • Western Blotting
  • Immunofluorescence
  • Immunoprecipitation (Native verified)

Note:

USA: RUO; Int'l: RUO