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Alfa Aesar™ CHAPS lysis buffer (2X)

Specifications

Chemical Name or Material CHAPS lysis buffer
MDL Number MFCD00282866
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
AAJ60812AC View Documents Alfa Aesar™
J60812AC
25mL N/A N/A
Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
AAJ60812AD View Documents Alfa Aesar™
J60812AD
50mL N/A N/A
Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Description

Description

Applications
CHAPS lysis buffe is used for cell lysis and protein extraction. CHAPS lysis buffer ideal for general cell-tissue lysis and subcellular orgnalle or molecular fractionation. CHAPS is recommended for the preparation of cytoplasmic cell lysates to be used with our caspase signaling pathway antibodies. The addition of calcium chloride to the CHAPS reagent preserves calcium dependent protein-protein interaction, whereas the addition of EDTA abrogates the interaction. Chaps can be used with tissues as well as culture cells, effective as a stand alone reagent for cell lysis and protein extraction as well as for immunoprecipitation,

Solubility
Fully misicible in water.

Notes
Contains 100mM Tris-HCl (pH 7.4), 220mM NaCl, 10mM EDTA and 2% CHAPS
Specifications

Specifications

CHAPS lysis buffer
MFCD00282866