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CDK9/Cyclin T1 Recombinant Human Protein DFS Item

CDK9/Cyclin T1 Recombinant Human Protein

Manufacturer:  Life Technologies LSPV4131

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Catalog No. PV4131



Description

Description

CDK9 is a serine⁄threonine kinase which when complexed with CyclinT1 forms an active holoenzyme complex. This complex plays a key role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1.
Specifications

Specifications

NP_001252 I NP_001231
Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis,
46.9/84.8kDa
50% by SDS-PAGE
–80°C
1 Each
CDK9/Cyclin T1
CDK9/cyclin T1 was pre-diluted in enzyme dilution buffer and assayed in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β−glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 100 μM ATP, 333μg/mL CDK7/9tide peptide substrate (YSPTSPSYSPTSPSYSPTSPSKKKK) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinases and inhibitors
CDK and CHK kinase family
35nmoles of phosphate transferred to CDK7/9tide peptide substrate (YSPTSPSYSPTSPSYSPTSPSKKKK) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 3.33μg/mL
His Tag
0.39mg/mL
1025, 4772, 4773, 904
CDK9/Cyclin T1 Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
CDK9/Cyclin T1 Recombinant Human Protein
Kinase assay
Baculovirus
CDK9/Cyclin T1 Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.