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CDK9/Cyclin K Recombinant Human Protein DFS Item

CDK9/Cyclin K Recombinant Human Protein

Manufacturer:  Life Technologies LSPV4335

Catalog No. PV4335



Description

Description

CDK9 is a serine⁄threonine kinase that phosphorylates the c-terminal domain of the largest subunit of RNA polymerase II and is involved in cell cycle regulation, This protein is coexpressed and copurified with CyclinK.
Specifications

Specifications

NP_001252, NP_003849
Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis, target and lead identification and validation
46.9/44.9, 91.8kDa
70% by SDS-PAGE
–80°C
1 Each
CDK9/cyclin K, TAK
CDK9/cyclin K was pre-diluted in enzyme dilution buffer and assayed in 25mM MOPS (pH 7.2), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 100μM ATP, 200μg/mL CHKtide substrate peptide (KKKVSRSGLYRSPSMPENLNRPR) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
CDK and CHK
2nmoles of phosphate transferred to CDK7/9tide peptide substrate (YSPTSPSYSPTSPSYSPTSPSKKKK) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 5μg/mL
His Tag
0.18mg/mL
1025, 8812
CDK9/Cyclin K Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
CDK9/Cyclin K Recombinant Human Protein
Kinase assay
Baculovirus
CDK9/Cyclin K Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.