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CDK6/Cyclin D1 Recombinant Human Protein DFS Item

CDK6/Cyclin D1 Recombinant Human Protein

Manufacturer:  Life Technologies LSPV4401

 View more versions of this product

Catalog No. PV4401



Description

Description

CDK6 functions as a cell-cycle initiator protein. This protein is coexpressed and copurified with CyclinD1.
Specifications

Specifications

NP_001250, NP_444284
Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis, target and lead identification and validation
CDK6/Cyclin D1 Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
CDK6/Cyclin D1 Recombinant Human Protein
Kinase assay
Baculovirus
CDK6/Cyclin D1 Recombinant Human Protein
Human
0.44mg/mL
66.4/61.2kDa
95% by SDS-PAGE
–80°C
1 Each
CDK6/cyclin D1, CCND1
CDK6/cyclin D1 was pre-diluted in enzyme dilution buffer and assayed in 25mM HEPES (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 1000μg/mL RbING peptide substrate (INGSPRTPRRGQNR) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
CDK and CHK
1,780nmoles of phosphate transferred to RbING peptide substrate (INGSPRTPRRGQNR) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 8.33μg/mL
GST Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.