Invitrogen™ CDK2/cyclin A1 Recombinant Human Protein
CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. Recombinant full-length human CDK2 and CyclinA1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Manufacturer: Invitrogen™ LSPV6291
CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition.
- Validated in LanthaScreen™ Kinase Binding Assay
- Protocols available for simple use and optimized performance
- Multiple pack sizes available for convenient use
|50mM Tris-HCl pH 7.5, 150mM NaCl, 0.25mM DTT, 10mM glutathione, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol|
|CDK2: p33(CDK2) CyclinA1: CCNA1|
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|Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.|