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Invitrogen™ CDC7/DBF4 Recombinant Human Protein DFS Item

CDC7 is a homolog protein that is critical for the G1/S transition and is also essential for initiation of DNA replication as cell division occurs. Recombinant full-length human CDC7 and DBF4 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tags.

Manufacturer:  Invitrogen™ LSPV6276

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Catalog No. PV6276



Description

Description

CDC7 (cell division cycle 7) is expressed in many normal tissues, but the overexpression of CDC7 may be associated with neoplastic transformation for some tumors and transformed cell lines. CDC7/DBF4 kinase promotes S phase by alleviating an inhibitory activity in Mcm4 that evolved to integrate several protein kinase.

  • Validated in LanthaScreen™ Kinase Binding Assay
  • Protocols available for simple use and optimized performance
  • Multiple pack sizes available for convenient use
Specifications

Specifications

Target & Lead Identification & Validation|Kinase Biology|Kinase Proteins & Expression Reagents|Protein Biology|Enzyme & Protein Activity Assays|Kinase Activity|Protein Assays and Analysis|Industrial & Applied Science|Pharma & Biopharma
91.2/103.5kDa
Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
Human
GST Tag
50mM Tris-HCl pH 7.5, 150mM NaCl, 0.25mM DTT, 10mM glutathione, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol
1mg
Kinase Assay
Yes
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