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CD90.2 (Thy-1.2) Rat anti-Mouse, eFluor® 450, Clone: 53-2.1, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS48090282

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Catalog No. 501128702



Description

Description

Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells. Applications Reported: This 53-2.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.015 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer′s cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Specifications

Specifications

CD90.2 (Thy-1.2)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01831
CD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2,Thy 1.2; thy-1 antigen; thy-1 membrane glycoprotein
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
53-2.1
eFluor® 450
Thy1
Liquid
Thy1
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
21838
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For Research Use Only.