CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), eFluor™ 450, eBioscience™, Invitrogen™

Description

Description: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. Expression level differences have been observed in different mouse strains. Applications Reported: This R17217 (RI7 217.1.4) antibody has been reported for use in flow cytometric analysis. Applications Tested: This R17217 (RI7 217.1.4) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD71 (Transferrin Receptor) Monoclonal antibody specifically detects CD71 (Transferrin Receptor) in Mouse samples. It is validated for Flow Cytometry.