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CD69 Armenian Hamster anti-Mouse, APC, Clone: H1.2F3, eBioscience™

Armenian Hamster Monoclonal Antibody

Manufacturer:  Life Technologies LS17069180

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Catalog No. 501129479



Description

Description

Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation. Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of stimulated mouse splenic cell cultures. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.
Specifications

Specifications

CD69
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P37217
AIM, BL-AC/P26, CLEC2C, EA1, GP32/28, MLR-3, C-type lectin domain family 2, member C, CD69 antigen
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
H1.2F3
APC
Cd69
Liquid
Cd69
IgG
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12515
Documents

For Research Use Only.