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CD45 Monoclonal Antibody (HI30), PE-Cyanine5.5, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
$182.00 - $182.00
Specifications
| Antigen | CD45 |
|---|---|
| Clone | HI30 |
| Concentration | 5 μL/Test |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|
501123547
|
Invitrogen
35045941 |
25 Tests |
Each for $182.00
|
|
|||||
Description
Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
CD45 Monoclonal antibody specifically detects CD45 in Human samples. It is validated for Flow Cytometry.Specifications
| CD45 | |
| 5 μL/Test | |
| Monoclonal | |
| Liquid | |
| RUO | |
| PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
| B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T | |
| PTPRC | |
| Primary | |
| 4° C, store in dark, DO NOT FREEZE! | |
| PTPRC |
| HI30 | |
| Flow Cytometry | |
| PE-Cyanine5.5 | |
| Mouse | |
| Human | |
| P08575 | |
| 5788 | |
| IgG1 κ | |
| Affinity chromatography | |
| Antibody |
For Research Use Only.