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CD366 (TIM3) Rat anti-Mouse, PE-Cy7, Clone: RMT3-23, eBioscience™

Rat Monoclonal Antibody

$158.05 - $390.05

Specifications

Antigen CD366 (TIM3)
Clone RMT3-23
Host Species Rat
Gene Alias TIM-3, HAVCR2, T cell immunoglobulin domain, mucin-like domain
Species Reactivity Mouse
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501123399

 
affymetrix
25587080
25μg Each for $158.05

501123400

 
affymetrix
25587082
100μg Each for $390.05
Description & Specifications

Specifications

Antigen CD366 (TIM3)
Clone RMT3-23
Host Species Rat
Gene Alias TIM-3, HAVCR2, T cell immunoglobulin domain, mucin-like domain
Species Reactivity Mouse
Applications Flow Cytometry
Regulatory Status RUO
Conjugate PE-Cy7
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.2mg/mL

The RMT3-23 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. The RMT3-23 antibody reacts with CD366 protein expressed by both BALB/c and C57BL/6 strains of mice. CD366, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. CD366 is expressed selectively by differentiated CD4+Th1 and CD8+Tc1 cells, but is absent on CD4+Th2 and CD8+Tc2 cells. Other hematopoietic cell types, including naïve T cells, B cells, macrophages and dendritic cells, do not express CD366, at least at the protein level. CD366 expression is upregulated at a late stage of T cell differentiation on Th1 cells after 3 rounds of in vitro polarization suggesting a role for this molecule in the transport or effector function of Th1 cells rather than a contribution to T cell differentiation. In an experimental autoimmune encephalomyelitis (EAE) model, CD366 was shown to be expressed on most CD4+ and CD8+ T cells in the central nervous system at the onset of clinical signs of disease, while less than 2% of CD4+ cells in the periphery expressed CD366 after immunization.

RMT3-23 has been shown to have functional activity; blocks DC recognition of apoptotic cells and also induces autoantibody production.