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CD366 (TIM3), APC, clone: 8B.2C12, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS17587180

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Catalog No. 501128590



Description

Description

HAVCR2 is a 281 A.A Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. It consists of an IgV-like domain and a mucin-like domain in the extracellular region and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. HAVCR2 plays an important role in the regulation of macrophage activation and induction of autoimmune diseases. It down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of HAVCR2 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease.

The 8B.2C12 antibody binds to the BALB/c allele of CD366 while reactivity to the C57Bl/6 allele is significantly weaker. Applications Reported: This 8B.2C12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 8B.2C12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.
Specifications

Specifications

CD366 (TIM3)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q8VIM0
HAVCR2,TIM-3
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
8B.2C12
APC
Havcr2
Liquid
Havcr2
IgG1, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
171285
Documents

For Research Use Only.