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CD357 (AITR/GITR) Rat anti-Mouse, PE, Clone: DTA-1, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS12587482

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Catalog No. 501129646



Description

Description

Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predomitly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease. Applications Reported: The DTA-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.004 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Glucocorticoid-induced tumor necrosis factor receptor family-related gene (GITR) is a member of the tumor necrosis factor receptor (TNFR) family that is expressed at low levels on unstimulated T cells, B cells, and macrophages. Upon activation, CD4+ and CD8+ T cells up-regulate GITR expression, whereas immunoregulatory T cells constitutively express high levels of GITR.
Specifications

Specifications

CD357 (AITR/GITR)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O35714
AITR, GITR-D, TNFRSF18
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
DTA-1
PE
Tnfrsf18
Liquid
Tnfrsf18
IgG2b, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
21936
Documents

For Research Use Only.