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CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), Functional Grade, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Supplier: Invitrogen 16587481
Description
Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predomitly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease. Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis and immunohistochemistry of frozen mouse tissue. It has also been reported for use in functional assays. Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.03 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This DTA-1 antibody has been tested by immunohistochemistry of frozen tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.
Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Specifications
CD357 (AITR/GITR) | |
Monoclonal | |
1 mg/mL | |
PBS with no preservative; pH 7.2 | |
O35714 | |
Tnfrsf18 | |
Affinity chromatography | |
RUO | |
21936 | |
4° C | |
Liquid |
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen) | |
DTA-1 | |
Functional Grade | |
Tnfrsf18 | |
Activation-inducible TNFR family receptor; AITR; CD357; GITR; GITR-D; glucocorticoid-induced TNFR-related protein; PRO364; TNF receptor superfamily activation-inducible protein; TNF receptor superfamily member 18; TNFRSF18; Tumor necrosis factor receptor superfamily member 18; tumor necrosis factor receptor superfamily, member 18; UNQ319; UNQ319/PRO364 | |
Rat | |
50 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2b κ |
For Research Use Only.