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CD326 (EpCAM) Mouse anti-Human, Alexa Fluor® 488, Clone: MH99, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS53832641

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Catalog No. 501129021



Description

Description

Description: The monoclonal antibody MH99 recognizes human CD326 also known as EpCAM (Epithelial cell adhesion molecule). This 40 kDa type I transmembrane protein is involved in cell-cell interactions in a calcium-independent manner. EpCAM is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of CD326, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. CD326 has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM. Based on crossblocking studies, MH99 and 1B7 see overlapping epitopes. Applications Reported: This MH99 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MH99 antibody has been pre-titrated and tested by flow cytometric analysis of the A549 cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Ep-CAM is a ∽40 kD glycoprotein, which functions as an epithelial cell adhesion molecule. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is expressed in the majority of human carcinomas and is a marker for tumors of epithelial lineage.
Specifications

Specifications

CD326 (EpCAM)
Monoclonal
5 μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P16422
Epithelial Specific Antigen, ESA, KSA, M4S1, MK-1, DIAR5, EGP-2, EGP40, KS1/4, MIC18, TROP1, TACSTD1, EPCAM, Epithelial cell adhesion molecule
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
MH99
Alexa Fluor® 488
EPCAM
Liquid
EPCAM
IgG2a, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
4072
Documents

For Research Use Only.