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CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eFluor™ 660, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Supplier: Invitrogen 50324982
Description
Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton. Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintece of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers. E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided. The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells. Applications Reported: This DECMA-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This DECMA-1 antibody has been tested by flow cytometric analysis of MDCK cell line. Optimal staining is achieved by intracellular staining as protein turnover can result in variable surface staining.
CD324 (E-Cadherin) Monoclonal antibody specifically detects CD324 (E-Cadherin) in Canine, Human, Mouse samples. It is validated for Flow Cytometry.Specifications
CD324 (E-Cadherin) | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
F1PAA9, P09803, P12830 | |
CDH1 | |
Affinity chromatography | |
RUO | |
12550, 442858, 999 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
DECMA-1 | |
eFluor 660 | |
CDH1 | |
AA960649; ARC-1; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, epithelial; cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; CDHE; cell adhesion molecule; cell-CAM 120/80; ECAD; E-cad; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; Ecadherin; E-cadherin; E-cadherin 1; E-cadherin precursor; Epithelial cadherin; hab; half baked; LCAM; L-CAM; Um; UVO; Uvomorulin | |
Rat | |
100 μg | |
Primary | |
Canine, Human, Mouse | |
Antibody | |
IgG1 κ |
For Research Use Only.