CD32 Mouse anti-Human, PE, Clone: 6C4 (CD32), eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Life Technologies LS12032942
Description: This 6C4 monoclonal antibody reacts with human CD32 (also known as Fc gamma RII). This antibody recognizes two isoforms of the receptor, Fc gamma RIIA and Fc gamma RIIB. These 40-kDa polymorphic transmembrane glycoproteins are expressed on B cells, granulocytes, monocytes, macrophages, and platelets. Moreover, these receptors are detected on natural killer cells. CD32 enables interaction between Fc gamma RII-expressing cells and opsonized antigen or IgG-containing immune complexes. Both receptors exhibit low affinity towards IgG and play a role in inflammation and autoimmune disease. This clone has been reported to inhibit Ig binding in a rosette blocking assay. Applications Reported: This 6C4 (CD32) antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6C4 (CD32) antibody has been pre-titrated and tested by flowcytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|RP11-5K23.6, CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, FcGR, IGFR2, MGC23887, MGC30032|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.