CD304 (Neuropilin-1), PerCP-eFluor™ 710, clone: TNKUSOHA, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Life Technologies LS46304941
Neuropilin 1 is a 923 amino acid containing membrane bound coreceptor to a tyrosine kinase receptor belonging to the neuropilin family with two CUB domains, two F5/8 type C domains and a MAM domain. Neuropilin 1 forms a heterodimer with NRP2 (Neuropilin 2) and is known to bind to plexin (PLXNB1) to form semaphorin receptors. Expressed mostly in the nervous system this receptor is implicated in a wide range of functions including in the development of the cardiovascular system, angiogenesis, repulsive axon guidance, formation of certain neuronal circuits, cell survival, control of neuronal migration and organogenesis outside the nervous system. This semaphorin receptor functions as receptors for both the semaphorins and vascular endothelial growth factor and is also a regulator of VEGF-induced angiogenesis. Neuropilin 1 may be involved in aggressive advanced prostate carcinoma and in the progression of breast cancer.Description: The TNKUSOHA monoclonal antibody recognizes human CD304, which is also known as BDCA-4 and Neuropilin-1. Like CD303, the CD304 antigen is expressed on human plasmacytoid dendritic cells in normal blood and bone marrow. CD304 can also be detected on T follicular helper cells in inflamed tonsils, as well as on some B-cell acute lymphoblastic leukemias. In addition, CD304 is expressed on numerous non-hematopoietic cell types, including neurons, endothelial, and tumor cells. Plasmacytoid dendritic cells play a key role in the innate immune response against viral pathogens and tumors.Applications Reported: This TNKUSOHA antibody has been reported for use in flow cytometric analysis.Applications Tested: This TNKUSOHA antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|BDCA4, CD304, NP1, NRP, VEGF165R, neuropilin-1, transmembrane receptor, vascular endothelial cell growth factor 165 receptor|
|IgG1, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.|
For Research Use Only.