CD300f (IREM-1) Mouse anti-Human, eFluor® 660, Clone: UP-D1, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Life Technologies LS50300842
Description: The monoclonal antibody recognizes human IREM-1 (Immune receptor expressed by myeloid cell 1). IREM-1 is also a member of of the CD300 family specifically referred to as CD300f. IREM-1 is a type I transmembrane protein with a single Ig domain, one predicted N-linked glycosylation site and a long cytoplasmic tail which contains a predicted ITIM domain categorizing it as an inhibitory receptor. Additionally the cytoplasmic domain has been shown to interact with SHP-1 and the p85\alpha subunit of PI3K. Expression is restricted to myeloid cells. In peripheral blood IREM-1 is found on monocytes and granulocytes. In monocyte derived dendritic cells, expression of IREM-1 is downregulated. Applications Reported: This UP-D1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UP-D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. CLM1 is an inhibitory receptor of the Ig superfamily expressed on myeloid cells. It mediates negative regulatory signals by recruiting SHP1 (PTPN6; MIM 176883) or SHIP.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CD300f, CLM-1, CLM1, IREM-1, IREM1, IgSF13, NKIR, CD300 antigen like family member F, CD300 antigen-like family member F, CD300 molecule like family member f, CMRF35-like molecule 1, NK inhibitory receptor, immune receptor expressed on myeloid cells 1, immunoglobin superfamily member 13, immunoglobulin superfam|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.