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CD289 (TLR9), APC, clone: eB72-1665, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS17909982

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Catalog No. 501128631



Description

Description

The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

Human Epithelial Cell lines were also reported to stain with this mAb (J. Immunol. 173: 1219). Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry. Applications Reported: This eB72-1665 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eB72-1665 antibody has been tested by intracellular staining and flow cytometric analysis of human TLR9-transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
Specifications

Specifications

CD289 (TLR9)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9NR96
Toll-like Receptor 9; Toll-like receptor 9 protein; UNQ5798/PRO19605
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
eB72-1665
APC
TLR9
Liquid
TLR9
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
54106
Documents

For Research Use Only.