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CD283 (TLR3) Mouse anti-Human, PE, Clone: TLR3.7, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS12903980

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Catalog No. 5011247



Description

Description

Description: The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). To date, at least twelve members of the Toll-like receptor family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. In the innate immune response, TLRs recognize molecular patterns associated with microbial pathogens and induce antimicrobial activity. TLR3 recognizes double-stranded (ds)RNA, induces the activation of NF-kappaB through MyD88-dependent and -independent pathways, and the production of type I interferons (IFNs). TLR3.7 suppressed poly(I):poly(C)-mediated IFN-beta production by human fibroblasts naturally expressing TLR3 on their surface. Applications Reported: The TLR3.7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TLR3.7 antibody has been tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines to detect the very low level of this antigen on the surface. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. TLR3 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression.
Specifications

Specifications

CD283 (TLR3)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O15455
Toll-like Receptor 3, IIAE2
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
TLR3.7
PE
TLR3
Liquid
TLR3
IgG1, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
7098
Documents

For Research Use Only.