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CD276 (B7-H3) Monoclonal Antibody (7-517), PE-Cyanine7, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Invitrogen 25276942
Description
Description: This 7-517 monoclonal antibody reacts with human CD276, which is also known as B7-H3. This type I transmembrane protein is expressed on immature and mature dendritic cells but not monocytes, granulocytes, or resting lymphocytes. Moreover, CD276 is expressed on non-hematopoietic cells such as osteoblasts, fibroblasts, and epithelial cells. However, CD276 expression can be induced on dendritic cells, monocytes, T, B, and NK cells. This co-stimulatory molecule binds activated T cells, leading to cell proliferation and IFN-gamma production. Applications Reported: This 7-517 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 7-517 antibody has been pre-titrated and tested by flow cytometric analysis of human monocyte-derived dendritic cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.
CD276 (B7-H3) Monoclonal antibody specifically detects CD276 (B7-H3) in Human samples. It is validated for Flow Cytometry.Specifications
CD276 (B7-H3) | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
Q5ZPR3 | |
CD276 | |
Affinity chromatography | |
RUO | |
80381 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
7-517 | |
PE-Cyanine7 | |
CD276 | |
4Ig-B7-H3; 6030411F23Rik; AU016588; B7 homolog 3; B7h3; B7-H3; B7RP-2; CD276; CD276 antigen; CD276 molecule; CD276 molecule tv1; costimulatory molecule; PSEC0249; sB7 H3; sB7H3; sCD276; soluble B7 H3; soluble B7H3; soluble CD276; UNQ309/PRO352 | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
For Research Use Only.