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CD275 (B7-H2) Monoclonal Antibody (HK5.3), eFluor™ 660, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Supplier: Invitrogen 50598582
Description
Description: The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of ∽40 kDa and belongs to the Ig superfamily. Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway. Applications Reported: This HK5.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
CD275 (B7-H2) Monoclonal antibody specifically detects CD275 (B7-H2) in Mouse samples. It is validated for Flow Cytometry.Specifications
CD275 (B7-H2) | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Q9JHJ8 | |
Icosl | |
Affinity chromatography | |
RUO | |
50723 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
HK5.3 | |
eFluor 660 | |
Icosl | |
AU044799; B7 homolog 2; B7 homologue 2; B7h; B7H2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP1; B7RP-1; BG071784; CD275; GI50; GL50; GL50-B; ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell costimulator ligand; inducible T-cell co-stimulator ligand; KIAA0653; LICOS; Ly115l; mKIAA0653; RGD1562791; transmembrane protein B7-H2 ICOS ligand | |
Rat | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.